CAM Microcirculation – Resonance Scanning Microscopy

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The dynamics of the hematogenous microcirculation of the CAM (as seen in this video) is a big reason why we use this model to understand cancer cell extravasation and cancer microparticle (extracellular vesicle) biogenesis.  To get the blue nuclei, I injected diluted Hoechst.  To get the endothelial/vessel walls, I injected LCA lectin-FITC.  To get the lumen, I injected 70kDa Alexa555-Dextran.  The merge panel (top) beautifully shows all the components in this highly active microcirculation environment.  Paper to come out on this very soon.

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